GSM2324110: Pax7 ChIPseq in AtT-20 expressing ER-Pax7 (no ER-Pax7 induction); Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Pax7
Cell type
Cell type Class
Neural
Cell type
AtT-20
Tissue
Pituitary
Disease
Tumor
Attributes by original data submitter
Sample
source_name
AtT-20 cells expressing chimera of 3x flag-ER-Pax7
cell type
pituitary corticotroph cell
passages
10-20
cell line
AtT-20 cells
treatment
3 days of 0.1% ethanol
chip antibody
Pax7 3xflag Sigma F1365
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei lysates were sonicated and protein-DNA complexes were isolated with specific antibodies. Fragments of 200-400bp were kept for library preparation Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina Hi-seq 2000 following the manufacturer's protocols to obtain 50bp paired end reads.