Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Pax7

Cell type

Cell type Class
Neural
Cell type
AtT-20
Tissue
Pituitary
Disease
Tumor

Attributes by original data submitter

Sample

source_name
AtT-20 cells expressing chimera of 3x flag-ER-Pax7
cell type
pituitary corticotroph cell
passages
10-20
cell line
AtT-20 cells
treatment
3 days of 0.1% ethanol
chip antibody
Pax7 3xflag Sigma F1365

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei lysates were sonicated and protein-DNA complexes were isolated with specific antibodies. Fragments of 200-400bp were kept for library preparation Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina Hi-seq 2000 following the manufacturer's protocols to obtain 50bp paired end reads.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
80583025
Reads aligned (%)
95.6
Duplicates removed (%)
9.4
Number of peaks
363 (qval < 1E-05)

mm9

Number of total reads
80583025
Reads aligned (%)
95.4
Duplicates removed (%)
9.9
Number of peaks
427 (qval < 1E-05)

Base call quality data from DBCLS SRA